WebApr 18, 2024 · Human alveolar macrophages simultaneously express M1 and M2 markers in steady state. Data from the two human cohorts from distinct geographical locations, UK (Additional file 1 Table S1) and Malawi (Additional file 1 Table S2), were collected independently and their comparison revealed a similar pattern of surface marker …
Proteoglycan-4 is an essential regulator of synovial macrophage ...
WebApr 12, 2024 · Dysregulated macrophage polarization and wound healing in diabetic mice. A Schematic illustration of wound healing and macrophage polarization.B … WebAug 25, 2024 · Baicalein promoted M1 macrophage polarization in vitro. THP-1-derived macrophages were treated with LPS and IFN-γ with or without different concentrations of baicalein for 24 h or 48 h. (A) the expression of CD86, the M1 macrophage marker, was quantified by flow cytometry. FlowJo software V10.0 was used for analyzing the mean … businesses temporarily closed
Human alveolar macrophages predominately express combined classical M1 ...
WebJun 11, 2013 · BHA had no effect on the increase of M1 macrophage marker CD86, while it blocked the expression of M2 macrophage marker CD163 14,27 (Figure 1B and 1C). Figure 1 BHA blocks M2 but not M1 … WebFlow cytometric analysis was conducted to detect the M1 macrophages stained with anti‑human CD86‑PE and anti‑human CD80‑FITC. M1 macrophages were calculated … MDM differentiation and polarization were performed slightly modifying the protocol described by Mantovani et al.46. Buffy coats from male healthy donors were obtained at the Blood Donor Centre at Policlinico Universitario Campus Bio-Medico after written informed consent, according to the institutional guidelines. … See more MDMs were fixed in 4% PFA for 10 min and permeabilised with 0.1% Triton X-100 (Sigma-Aldrich). After washing, cytoskeletal actin … See more Gene expression levels were evaluated by two-step Quantitative Reverse Transcription PCR (RT-qPCR). Isolation and purification of mRNA were performed using TRI Reagent (Sigma-Aldrich). Extracted mRNA was … See more MDMs were stained with monoclonal mouse anti-human CD68, CD80, CD86, CD163, and CD206 (mannose receptor, MR) antibodies (eBioscience, Affymetrix). Cells were suspended in PBS at a concentration of 1 × … See more Statistical analysis of flow cytometric and gene expression data was performed using GraphPad Prism ver. 6.0 suite (GraphPad Software). Conditions of normality were … See more businesses technology